Biosensors for Measuring Metastatic Potential and Chemoresistance of Single Cancer Cells
AssigneeThe Regents of the University of California
InventorPradipta Ghosh
Life of this patent
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Abstract
The present invention provides a Fluorescence Resonance Energy Transfer (FRET) biosensor that measures the metastatic potential and/or chemoresistance of single living cancer cells, and/or cells associated with fibrogenesis, diabetes, aging, and/or infertility.
Claims15
A method for detecting metastatic potential and/or chemoresistance in single cells, comprising;
- providing one or more multi-modular biosensors;
- providing a sample comprising a cell;
- contacting said sample with the one or more biosensors under conditions that allow folding of probe donor and acceptor fluorescent proteins into close proximity;
- and
- detecting fluorescence resonance energy transfer (FRET) signal to detect said interacting molecules.
A method for identifying agents specifically targeting a GNAI:GIV interface, comprising;
- providing one or more multi-modular biosensors;
- providing a sample comprising a cell;
- contacting said sample with the one or more biosensors to form a first mixture under conditions that allow the folding of probe donor and acceptor fluorescent proteins into close proximity;
- detecting a first FRET signal to detect said interacting molecules;
- contacting said first mixture with an agent under said conditions that allow the folding of probe donor and acceptor fluorescent proteins into close proximity;
- detecting a second FRET signal to detect said interacting molecules;
- and
- determining the difference between said first and said second FRET signal.
A biosensor device for detecting metastatic potential and/or chemoresistancy of single cells comprising a biosensor that targets short stretches wherein the one or more multi-modular biosensors target short stretches of the C-terminus of GIV flanking one or both of tyrosines, Y1764 and Y1798, and N-SH2 domain of p85α(PI3K).
Dependencies are inferred from the claim text.