Target substance separation method and quantification method
AssigneeTerumo Kabushiki Kaisha
InventorHiroya SATO
Life of this patent
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Abstract
A method for separating a target substance includes: forming a mixture containing: a target substance-magnetic particle complex that includes: a sample containing a target substance, and magnetic particles to which a first receptor is fixed, wherein the first receptor is adapted to specifically recognize a site of the target substance; and separating the target substance-magnetic particle complex from the mixture by magnetism and electrophoresis.
Claims11
A method for separating a target substance, the method comprising:
- forming a mixture containing:
- a target substance-magnetic particle complex that comprises:
- a sample containing a target substance, and
- magnetic particles to which a first receptor is fixed, wherein the first receptor is adapted to specifically recognize a site of the target substance;
- and
- separating the target substance-magnetic particle complex from the mixture by magnetism and electrophoresis.
A method for measuring a target substance amount, the method comprising:
- forming a mixture containing:
- a target substance-magnetic particle-labeled substance complex that comprises:
- a sample containing a target substance, and
- magnetic particles to which a first receptor is fixed, wherein the first receptor is adapted to specifically recognize a site of the target substance, and
- a labeled substance to which a second receptor is fixed, wherein the second receptor is adapted to specifically recognize a site different from the site of the target substance;
- separating the target substance-magnetic particle-labeled substance complex from the mixture by magnetism and electrophoresis;
- and
- subsequently, measuring the target substance amount in the sample, which comprises detecting a signal intensity of the target substance-magnetic particle-labeled substance complex.
An inspection kit for a target-substance-related disease comprising:
- magnetic particles to which a first receptor is fixed, wherein the first receptor is adapted to specifically recognize a site of a target substance;
- a labeled substance to which a second receptor is fixed, wherein the second receptor is adapted to specifically recognize a site different from the site that is recognized by the target substance;
- and
- a magnet.
Dependencies are inferred from the claim text.