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US 2021/0239664 A1Published application
Exosome production method
AssigneeMie University
InventorsHiroshi Shiku, Naohiro Seo, Tsuguhiro Kaneda, Junko Nakamura, Kazunari Akiyoshi
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Abstract
The present invention aims to provide a method for preparing exosomes comprising: (i) a step for ultrafiltering a sample containing at least one exosome; and (ii) a step for subjecting the sample that can be obtained from step (i) to anion exchange column chromatography.
Claims37
An exosome production method comprising:
- (i) a step for ultrafiltering a biological sample containing exosomes;
- and (ii) a step for subjecting the sample that can be obtained from step (i) to anion exchange column chromatography.
A method for preparing exosomes from a biological sample, the method comprising:
- (i) a step for performing centrifugal separation for a biological sample to remove contaminants from the sample, and then obtaining the supernatant;
- (ii) a step for subjecting the supernatant obtained in the step (i) to ultrafiltration, and then concentrating the supernatant;
- and (iii) a step for subjecting the concentrated liquid obtained in the step (ii) to anion exchange column chromatography.
A system for preparing exosomes from a biological sample, which comprises anion exchange column chromatography.
Dependencies are inferred from the claim text.