Lc/ms adduct mitigation by vapor deposition coated surfaces
AssigneeWaters Technologies Corporation
InventorsKerri M. Smith, Paul Rainville
Life of this patent
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Abstract
The present disclosure discusses a method of separating a sample (e.g., small organic acid metabolite) including coating a flow path of a chromatographic system. The coating along the flow path is vapor deposited and prevents or severely decreases metal interactions between the metallic chromatographic system and the sample.
Claims20
A method of separating and analyzing a sample comprising:
- injecting the sample into a chromatographic system having a fluid-contacting coating on a metallic surface, wherein the coating comprises an alkylsilyl, and the sample comprises one or more tricarboxylic acid cycle metabolites;
- flowing the injected sample through the chromatographic system;
- separating the flowing sample with the chromatographic system;
- and
- analyzing the separated sample with a mass spectrometer.
A method of increasing resolution of a sample comprising one or more tricarboxylic acid cycle metabolites when using a chromatographic system, the method comprising:
- providing the chromatographic system having a fluid-contacting coating on a metallic surface, wherein the coating comprises an alkylsilyl;
- injecting the sample into the chromatographic system;
- flowing the injected sample through the chromatographic system;
- separating the flowing sample with the chromatographic system;
- and
- passing the separated sample through a mass spectrometer to analyze the separated sample.
A method of studying etiology of a disease, the method comprising:
- injecting the sample into a chromatographic system having a fluid-contacting coating on a metallic surface, wherein the coating comprises an alkylsilyl, and the sample comprises one or more tricarboxylic acid cycle metabolites;
- flowing the injected sample through the chromatographic system;
- separating the flowing sample with the chromatographic system;
- and
- analyzing the separated sample with a mass spectrometer.
Dependencies are inferred from the claim text.