US 2021/0231941 A1 · MICROSCOPY METHOD AND MICROSCOPE FOR PRODUCING AN IMAGE OF AN OBJECT

Abstract

A microscopy method is for producing an electronic image of an object, wherein the object is imaged with an adjustable optical imaging scale on an image detector. The method includes: selecting a parameter for the electronic image, wherein the parameter can be influenced by the optical imaging scale and differs from the image field dimensions, and setting a setpoint value range for the parameter, setting a total imaging scale for the electronic image, wherein adjusting or controlling the parameter of the electronic image is implemented such that, at the same time, the parameter of the electronic image lies in the specified setpoint value range with a tolerance and the set total imaging scale is obtained, wherein the optical imaging scale forms a basis for a manipulated variable of the adjustment or closed-loop control and a digital image magnification is carried out on the basis of the set total imaging scale.

Claims22

Dependent claims

A microscopy method for producing an electronic image of an object, the method comprising:

setting a total imaging scale for the electronic image;
providing a specified parameter for the electronic image, wherein the specified parameter includes at least one of the following:
a signal-to-noise ratio in the electronic image, a depth of field range, a working distance being a distance between the microscope and the object, and an object illumination intensity;
specifying a setpoint value range for the specified parameter;
illuminating and imaging the object with an adjustable optical imaging scale onto an electronic image detector providing electronic image data while open-loop or closed-loop controlling the specified parameter to have a value in the specified setpoint value range with a tolerance, wherein at least the optical imaging scale forms a basis for a manipulated variable of the open-loop or closed-loop control;
and,
generating the electronic image by performing digital image magnification on the electronic image data to the total imaging scale.

4 more elements

A microscope for producing an electronic image of an object, the microscope comprising:

an image detector configured to generate electronic image data;
an objective lens, a tube lens and an adjustable optical zoom lens configured to conjointly produce an optical image on said image detector with an adjustable optical imaging scale;
a control device including a processor and being configured to produce an electronic image of the object from the electronic image data and to adjust the adjustable optical imaging scale;
said control device being further configured to:
set a total imaging scale for the electronic image,
provide at least one specified parameter for the electronic image, wherein the specified parameter includes at least one of:
a signal-to-noise ratio in the electronic image, a depth of field range, a working distance being a distance between the microscope and the object, and an object illumination intensity,
specify a setpoint value range for the specified parameter,
illuminate and image the object with the adjustable optical imaging scale onto the electronic image detector while open-loop or closed-loop controlling the specified parameter to have a value in the specified setpoint value range with a tolerance, wherein at least the optical imaging scale forms a basis for a manipulated variable of the open-loop or closed-loop control, and
generate the electronic image by performing digital image magnification on the electronic image data to the total imaging scale.

7 more elements

Dependencies are inferred from the claim text.

Microscopy method and microscope for producing an image of an object

Life of this patent

Microscopy method and microscope for producing an image of an object

Life of this patent

Abstract

Claims22