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US 2021/0231650 A1Published application
Method for electronic detection and quantification of antibodies
AssigneeArizona Board of Regents on behalf of Arzona State University
InventorsStuart LINDSAY, Bintian ZHANG
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Abstract
The present disclosure relates to a sensing device and methods for detecting, and quantifying the amount of, a target protein in a sample.
Claims8
A sensing device comprising:
- a first and a second electrode, the first and second electrode being separated by a gap and comprising an opening formed therethrough;
- wherein:
- a plurality of ligands is attached to at least one of the first electrode and the second electrode;
- the ligand has a length L1 and binds a target protein having a length L2;
- and
- the width of the gap is no less than 2L1 and no more than 2L1+L2.
3 more elements
A method of detecting the presence of a protein/antibody in a sensing device, the method comprising:
- recording a current when a sample suspected of comprising a target protein is in contact with the sensing device, wherein
- the sensing device comprises:
- a first and a second electrode, the first and second electrode being separated by a gap and comprising an opening formed therethrough;
- wherein:
- a plurality of ligands is attached to at least one of the first electrode and the second electrode;
- the ligand has a length L1 and binds a target protein having a length L2;
- and
- the width of the gap is no less than 2L1 and no more than 2L1+L2;
- a detector for measuring the current produced when the target protein binds the ligand;
- and determining that the protein/antibody is present by detecting an increase in current.
7 more elements
A method of determining the concentration of a target protein in a sample, the method comprising:
- recording the time course of current when a sample suspected of comprising a protein/antibody is in contact with a sensing device, wherein
- the sensing device comprises:
- a first and a second electrode, the first and second electrode being separated by a gap and comprising an opening formed therethrough;
- wherein:
- a plurality of ligands is attached to at least one of the first electrode and the second electrode;
- the ligand has a length L1 and binds a target protein having a length L2;
- and
- the width of the gap is no less than 2L1 and no more than 2L1+L2;
- a detector for measuring the current produced when the target protein binds the ligand;
- and determining the concentration of protein/antibody from the time course of current.
7 more elements
Dependencies are inferred from the claim text.