Reagents for potentiometric magnesium ion selective electrode sensors and methods of production and use thereof
AssigneeSiemens Healthcare Diagnostics Inc.
InventorsWei Zhang, Kevin Horan
Life of this patent
Estimating the term…
Abstract
Reagents are disclosed for use with potentiometric magnesium ion selective electrodes, along with kits containing same as well as methods of use thereof.
Claims20
A method of calibrating a solid-state planar magnesium sensing membrane for a potentiometric ion selective electrode that detects ionized magnesium in a biological sample, the method comprising:
- calibrating the magnesium sensing membrane with at least three separate calibration reagents, wherein each of the calibration reagents comprises calcium ions and magnesium ions present in a calcium:magnesium molar ratio in a range of from about 1.5:1 to about 3.25:1.
A method of calibrating a solid-state planar magnesium sensing membrane for a potentiometric ion selective electrode that detects ionized magnesium in a biological sample, the method comprising:
- calibrating the magnesium sensing membrane with at least three separate calibration reagents, wherein each of the calibration reagents comprises calcium ions and magnesium ions present in a calcium:magnesium molar ratio in a range of from about 1.5:1 to about 3.25:1, and wherein each of the calibration reagents further comprises a poly(ethylene oxide) surfactant selected from the group consisting of:
- (a) the poly(ethylene oxide) surfactant is represented by the structure of formula I:
- wherein n is in the range of from about 9 to about 10;
- (b) the poly(ethylene oxide) surfactant is represented by the structure of formula II:
- HO—(CH2—CH2—O—)23—C12H25 Formula II;
- and
- (c) the poly(ethylene oxide) surfactant is represented by the structure of formula III:
- wherein n is 100.
A method of calibrating a solid-state planar magnesium sensing membrane for a potentiometric ion selective electrode that detects ionized magnesium in a biological sample, the method comprising:
- calibrating the magnesium sensing membrane with at least three separate calibration reagents, wherein each of the calibration reagents comprises:
- calcium ions and magnesium ions present in a calcium:magnesium molar ratio in a range of from about 1.5:1 to about 3.25:1;
- a pH in a range of from about 6 to about 8;
- sodium ions at a concentration of at least 50 mmol/L;
- and
- a poly(ethylene oxide) surfactant selected from the group consisting of:
- (a) the poly(ethylene oxide) surfactant is represented by the structure of formula I:
- wherein n is in the range of from about 9 to about 10;
- (b) the poly(ethylene oxide) surfactant is represented by the structure of formula II:
- HO—(CH2—CH2—O—)23—C12H25 Formula II;
- and
- (c) the poly(ethylene oxide) surfactant is represented by the structure of formula III:
- wherein n is 100;
- contacting the magnesium sensing membrane with at least one additional reagent selected from a wash reagent and a quality control reagent, wherein the at least one additional reagent comprises calcium ions and magnesium ions present in a calcium:magnesium molar ratio in a range of from about 1.5:1 to about 3.25:1;
- and
- wherein the magnesium sensing membrane is subjected to multiple calibrations with the at least three separate calibration reagents and is stable for at least four weeks.
Dependencies are inferred from the claim text.