Modified factor ix polypeptides and uses thereof
AssigneeCatalyst Biosciences, Inc.
InventorsEdwin L. Madison, Christopher Thanos, Grant Ellsworth Blouse
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Abstract
Modified Factor IX (FIX) polypeptides and uses thereof are provided. Nucleic acid molecules encoding modified factor IX (FIX) polypeptides and uses thereof also are provided. Such modified FIX polypeptides include FIXa and other forms of FIX. Among the modified FIX polypeptides provided are those that have altered activities, typically altered procoagulant activity, including increased procoagulant activities. Hence, such modified polypeptides are therapeutics.
Claims43
A modified Factor IX (FIX) polypeptide, comprising one or more amino acid modification(s) in an unmodified FIX polypeptide, wherein:
- an amino acid modification corresponds to R318Y, R318E, R318F, or R318W, with reference to the sequence of amino acids set forth in SEQ ID NO:3;
- corresponding amino acid residues are identified by alignment of the unmodified FIX polypeptide with the FIX polypeptide of SEQ ID NO:3;
- the unmodified FIX polypeptide comprises a sequence of amino acids set forth in any of SEQ ID NOs:
- 2, 3, 20, or 325, or a sequence of amino acids having at least 95% sequence identity to the FIX polypeptide sequence set forth in any of SEQ ID NOs:
- 2, 3, 20, or 325, or a catalytically active fragment thereof;
- and
- modifications are amino acid replacements, additions, or deletions, or any combination thereof.
Dependencies are inferred from the claim text.