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US 2021/0230548 A1Published application
Natural killer cells engineered to express chimeric antigen receptors with immune checkpoint blockade
AssigneeBoard of Regents, The University of Texas System
InventorsMay Daher, Rafet Basar, Elizabeth Shpall, Katy Rezvani
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Abstract
Provided herein are methods for producing NK cells expressing chimeric antigen receptors and having no expression of CISH. Further provided are methods for treating diseases by administering the CAR NK cells.
Claims54
An isolated natural killer (NK) cell engineered to express (1) a chimeric antigen receptor (CAR) and/or a T cell receptor (TCR) and (2) human IL-15 (hIL-15) and to have essentially no expression of CISH.
A method for producing NK cells of any one of claims 1-14 comprising:
- (a) obtaining a starting population of NK cells;
- (b) culturing the starting population of NK cells in the presence of artificial presenting cells (APCs);
- (c) introducing a CAR and/or TCR expression vector into the NK cells;
- (d) expanding the NK cells in the presence of APCs, thereby obtaining expanded NK cells;
- and
- (e) disrupting the expression of CISH in the expanded NK cells.
3 more elements
Dependencies are inferred from the claim text.