Compositions and methods for increasing or enhancing transduction of gene therapy vectors and for removing or reducing immunoglobulins

A method of treating a subject in need of treatment for a disease caused by a gain of function activity or expression, of a protein comprising:

• (a) administering to said subject a recombinant viral vector comprising a heterologous polynucleotide that is transcribed into a nucleic acid that inhibits, decreases or reduces expression of the gain of function, activity or expression of said protein;
• and (b) administering to said subject a protease or glycosidase effective to degrade or digest and/or inhibit or reduce effector function of antibodies that bind to said recombinant viral vector.

A method of treating a subject in need of treatment for a disease caused by a loss of function or activity of a protein comprising:

• (a) administering to said subject a protease or glycosidase effective to degrade or digest and/or inhibit or reduce effector function of viral vector binding antibodies;
• and (b) administering to said subject a recombinant viral vector comprising a heterologous polynucleotide that encodes a protein or peptide that provides or supplements the function or activity of said protein.

A method of treating a subject in need of treatment for a disease caused by a gain of function activity or expression, of a protein comprising:

• (a) administering to said subject a protease or glycosidase effective to degrade or digest and/or inhibit or reduce effector function of viral vector binding antibodies;
• and (b) administering to said subject a recombinant viral vector comprising a heterologous polynucleotide that is transcribed into a nucleic acid that inhibits, decreases or reduces expression of the gain of function, activity or expression of said protein.

67-77. (canceled)

A method for treating a disease treated by a gene therapy vector comprising administering to a subject in need thereof a therapeutically effective amount of an immunoglobulin G-degrading enzyme polypeptide.