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AssigneesSumitomo Chemical Company, Limited, Riken
InventorsTokushige NAKANO, Satoshi ANDO, Yoshiki SASAI, Mototsugu EIRAKU
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The invention provides a method for producing a retinal tissue by (1) subjecting pluripotent stem cells to floating culture in a serum-free medium containing a substance inhibiting the Wnt signal pathway to form an aggregate of pluripotent stem cells, (2) subjecting the aggregate to floating culture in a serum-free medium containing a basement membrane preparation, and then (3) subjecting the aggregate to floating culture in a serumcontaining medium. The invention also provides a method for producing an optic-cup-like structure, a method for producing a retinal pigment epithelium, and a method for producing a retinal layer-specific neural cell.
An artificial cell aggregate comprising a retinal tissue, with Brn3 and TuJ1-positive ganglion cells in the lowermost layer of the retinal tissue, Crx and Recoverin-positive photoreceptor-precursor cells in the outermost layer and intermediate layer of the retinal tissue, and interneuron progenitor cells between the outermost layer and the lowermost layer of the retinal tissue.
Dependencies are inferred from the claim text.