Extracellular vesicles and methods of using
AssigneeBoard of Regents of the University of Nebraska
InventorJanos Zempleni
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to materials and methods for extracellular vesicle (e.g., exosome) -mediated delivery of cargo (e.g., endogenous and/or exogenous) to non-bovine mammalian (e g., human) cells. For example, exosomes isolated from bovine milk for delivering cargo to non-bovine mammalian (e.g., human) cells are provided.
Claims29
A milk exosome comprising a biological membrane surrounding a lumen, wherein said milk exosome comprises an exogenous microRNA (miRNA) encapsulated in said lumen, wherein said miRNA is biologically active in a mammal.
3-4. (canceled)
The milk exosome of claim 4, wherein said miRNA is selected from the group consisting of MiR-320a, MiR-34a, miR-155-5p, and miR-375.
6-8. (canceled)
17-72. (canceled)
74-75. (canceled)
77-78. (canceled)
80-84. (canceled)
A method of targeting a milk exosome to a selected mammalian cell or tissue, said method comprising administering a milk exosome to a mammal, wherein said milk exosome comprises a biological membrane surrounding a lumen, and wherein said milk exosome comprises an exogenous microRNA (miRNA) encapsulated in said lumen.
89-106. (canceled)
Dependencies are inferred from the claim text.