Cell-free dna for assessing and/or treating cancer
AssigneeThe Johns Hopkins University
InventorsVictor E. Velculescu, Stephen Cristiano, Alessandro Leal, Jillian A. Phallen, Jacob Fiksel, Vilmos Adleff, Robert B. Scharpf
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Abstract
This document relates to methods and materials for assessed, monitored, and/or treated mammals (e.g., humans) having cancer. For example, methods and materials for identifying a mammal as having cancer (e.g., a localized cancer) are provided. For example, methods and materials for assessing, monitoring, and/or treating a mammal having cancer are provided.
Claims21
A method of determining a cell free DNA (cIDNA) fragmentation profile of a mammal, the method comprising:
- processing cfDNA fragments obtained from a sample obtained from the mammal into sequencing libraries;
- subjecting the sequencing libraries to low-coverage whole genome sequencing to obtain sequenced fragments;
- mapping the sequenced fragments to a genome to obtain windows of mapped sequences;
- and
- analyzing the windows of mapped sequences to determine cfDNA fragment lengths.
A method of identifying a mammal as having cancer, the method comprising:
- determining a cell free DNA (cfDNA) fragmentation profile in a sample obtained from the mammal;
- comparing the cfDNA fragmentation profile to a reference cfDNA fragmentation profile;
- and
- identifying the mammal as having cancer when the cfDNA fragmentation profile obtained from the mammal is different from the reference cfDNA fragmentation profile.
21-67. (canceled)
Dependencies are inferred from the claim text.