Lysis buffers for extracting nucleic acids
AssigneeLife Technologies Corporation
InventorsJames STRAY, Jason Yingjie Liu, Maxim Brevnov, Jaiprakash Shewale, Allison Holt
Life of this patent
Estimating the term…
Abstract
The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods, and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material.
Claims30
1-12. (canceled)
A method of making a product, wherein the product comprises a nucleic acid, comprising:
- a) incubating a solid in a lysate solution, the solid comprising the nucleic acid, wherein the lysate solution comprises a detergent, a chelating agent, a reducing agent, and an enzyme;
- b) extracting a supernatant, wherein the supernatant comprises the nucleic acid extracted from the solid.
(canceled)
(canceled)
27-50. (canceled)
A method of separating a nucleic acid from a solid sample, comprising:
- (a) lysing the solid sample in a lysis solution, thereby forming a mixture comprising the nucleic acid, wherein the lysate solution comprises a detergent, a chelating agent, a reducing agent, and an enzyme;
- (b) centrifuging the mixture, wherein the nucleic acid is thereby separated from the solid sample.
52-53. (canceled)
The method of claim 53, wherein the solid is a biological material selected from bone, cartilage, ligament, tendon, and tooth.
The method of claim 53, wherein the solid is an adhesive material that comprises a biological sample.
(canceled)
58-59. (canceled)
61-67. (canceled)
A method of identification comprising:
- (a) lysing a sample in a lysis solution, thereby forming a lysate comprising a nucleic acid from the sample, wherein the lysate solution comprises a detergent, a chelating agent, a reducing agent, and an enzyme;
- (b) extracting the lysate, wherein the nucleic acid is in the lysate;
- (c) analyzing the nucleic acid;
- and
- (d) identifying a source of the nucleic acid of the sample based on the nucleic acid analysis.
(canceled)
72-100. (canceled)
Dependencies are inferred from the claim text.