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AssigneeUniversity Health Network
InventorsMaria Cristina NOSTRO, Farida SARANGI, Roman KORYTNIKOV, Emily MCGAUGH, Frankie Hoi Chun POON
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Methods and compositions are provided for producing PDX1+/NKX6-1+ pancreatic progenitor cells from a PDX1+ endodermal cell population. The method comprises contacting the endodermal cell population with an EGF component and tankyrase inhibitor that binds to an adenosine subsite of a tankyrase enzyme, to induce the differentiation of at least a portion of the PDX1+ endodermal cell population into PDX1+NKX6-1+ pancreatic progenitor cells.
A method of producing PDX1+/NKX6-1+ pancreatic progenitor cells from a PDX1+ endodermal cell population, the method comprising contacting the endodermal cell population with:
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A culture medium culture composition comprising a suitable base culture medium, EGF, a tankyrase inhibitor that binds to an adenosine subsite of a tankyrase enzyme, and optionally a BMP inhibitor.
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Dependencies are inferred from the claim text.