Composition and method for preventing or treating eye disorder
AssigneeBuddhist Tzu Chi Medical Foundation
InventorsYuan-Chieh Lee, Li-Yi Sun
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AssigneeBuddhist Tzu Chi Medical Foundation
InventorsYuan-Chieh Lee, Li-Yi Sun
Estimating the term…
Provided is a method for preparing mesenchymal stem cell-conditioned medium, including isolating mesenchymal stem cells from a subject, maintaining the mesenchymal stem cells in a mesenchymal stem cell maintenance medium, collecting the mesenchymal stem cells at passages 2 to 5, culturing the mesenchymal stem cells in a medium supplemented with fetal bovine serum and mesenchymal stem cell culture adjuvant for hours to obtain a mesenchymal stem cell-conditioned medium, harvesting the medium and centrifuging followed by filtering. Also provided is a method for preventing or treating epithelial tissue disorder of eyes such as dry eye syndrome, including applying the medium to an eye of a subject. Further provided is a medical composition including the medium.
A method for preventing or treating epithelial tissue disorder of eyes in a subject in need thereof, comprising administering to said subject a therapeutically effective amount of a bioactive formulation comprising a composition prepared by:
Dependencies are inferred from the claim text.