Method for producing active gcmaf
AssigneeFoundation for Biomedical Research and Innovation At Kobe
InventorsYo-ichi NABESHIMA, Yoko NABESHIMA, Chiaki ABE
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Abstract
The purpose of the present invention is to provide a method for producing active GcMAF more simply and at a high yield. The present invention is a method for producing active GcMAF comprising a step for culturing host cells which are transfected with a VDBP expression vector in serum-free medium. This culture is preferably a suspension culture. In addition, this method for producing active GcMAF is also characterized by not requiring an enzyme treatment step for deglycosylation.
Claims8
A method for producing active Gc protein-derived macrophage activating factor (GcMAF), comprising a step of culturing host cells transfected with a vitamin-D binding protein expression vector in a serum-free medium.
Dependencies are inferred from the claim text.