Scaleable inline buffer dilution scheme

An inline buffer dilution system, comprising:

• a vessel comprising a supply of diluent liquid;
• a first vessel comprising a supply of a first buffer, the first vessel pressurized using only atmospheric pressure;
• a second vessel comprising a supply of a second buffer, the second vessel pressurized using only atmospheric pressure;
• a first flow control valve arranged downstream of and configured to communicate with the supply of diluent liquid;
• a second flow control valve arranged downstream of and configured to communicate with the first vessel comprising the supply of the first buffer;
• a third flow control valve arranged downstream of and configured to communicate with the second vessel comprising the supply of the second buffer;
• a mixing pump fluidly connected to the first, second, and third flow control valves and configured to receive an amount of the diluent liquid, the first buffer, and the second buffer via the first, second, and third flow control valves, respectively, the mixing pump further configured to mix the amount of the diluent liquid, the first buffer, and the second buffer to produce a diluted buffer solution;
• a backpressure control valve arranged downstream of the mixing pump and configured to generate a backpressure that promotes mixing within the mixing pump;
• and
• a controller communicatively connected to the mixing pump and the backpressure control valve, the controller configured to control the backpressure generated by the backpressure control valve based on the amount of the diluent liquid, the first buffer, and the second buffer being mixed in the mixing pump, such that the mixing pump yields a minimum mixing threshold across a range of fluid flows.

A method of producing a diluted buffer solution across a range of fluid flows, comprising:

• providing a first flow control valve adapted to communicate with a vessel comprising a supply of diluent liquid, a second flow control valve adapted to communicate with a first vessel comprising a supply of a first buffer, and a third flow control valve adapted to communicate with a second vessel comprising a supply of a second buffer;
• directing an amount of the diluent liquid, the first buffer, and the second buffer to a mixing pump;
• mixing the amount of the diluent liquid, the first buffer, and the second buffer in the mixing pump, thereby producing a diluted buffer solution;
• generating, via a backpressure control valve arranged downstream of the mixing pump, a backpressure that promotes mixing within the mixing pump;
• and
• controlling, via a controller communicatively connected to the mixing pump and the backpressure control valve, the backpressure generated by the backpressure control valve based on the amount of the diluent liquid, the first buffer, and the second buffer being mixed in the mixing pump.