Method of Producing Lipid

A method of improving productivity of medium-chain fatty acids, comprising the steps of:

• culturing a transformant wherein a gene encoding the following protein (A) or (B), and a gene encoding the following protein (C) or (D) are introduced into a host cell, and
• improving the productivity of medium-chain fatty acids or lipids containing the same as components:
• (A) a protein consisting of the amino acid sequence set forth in SEQ ID NO:
• 2;
• (B) a protein consisting of an amino acid sequence having 60% or more identity with the amino acid sequence set forth in SEQ ID NO:
• 2, and having acyl-ACP thioesterase activity;
• (C) a protein consisting of the amino acid sequence set forth in SEQ ID NO:
• 4;
• and
• (D) a protein consisting of an amino acid sequence having 60% or more identity with the amino acid sequence set forth in SEQ ID NO:
• 4, and having β-ketoacyl-ACP reductase activity.

A transformant, wherein expression of a gene encoding the following protein (A) or (B), and expression of a gene encoding the following protein (C) or (D) is enhanced in a host cell:

• (A) a protein consisting of the amino acid sequence set forth in SEQ ID NO:
• 2;
• (B) a protein consisting of an amino acid sequence having 60% or more identity with the amino acid sequence set forth in SEQ ID NO:
• 2, and having acyl-ACP thioesterase activity;
• (C) a protein consisting of the amino acid sequence set forth in SEQ ID NO:
• 4;
• and
• (D) a protein consisting of an amino acid sequence having 60% or more identity with the amino acid sequence set forth in SEQ ID NO:
• 4, and having β-ketoacyl-ACP reductase activity.