Molecular imaging and related methods

A method of imaging single molecules, the method comprising:

• obtaining a sample that includes a plurality of complexes, a respective complex of the plurality of complexes including a target molecule bound to a probe, the probe including one or more fluorescent groups that interact with light at one or more wavelengths;
• exposing an area of the sample to one or more wavelengths of light that interact with the one or more fluorescent groups, wherein an interference pattern is generated on the area of the sample in a region of overlap between two or more coherent laser beams;
• and
• detecting a result from the interacting of the one or more wavelengths of light that interact with the one or more fluorescent groups using 20× objective magnification to provide an image of one or more single molecules without variation of any detection settings so that the image possesses a resolution better than 450 nm over the imaged area of at least 1×105 μm2.