Method for Using Electrochemical Bioreactor Module with Recovery of Cofactor
AssigneeBiocheminsights, Inc.
InventorsClifford S. Morrison, William B. Armiger, David R. Dodds, Mattheos Koffas
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Abstract
Provided herein are composition and process for using an electrochemical device for the reduction of the oxidized state of phosphorylated or non-phosphorylated nicotinamide adenine dinucleotide to the reduced state in which unwanted products of the electrochemical reduction are recovered as the oxidized state of the phosphorylated or non-phosphorylated nicotinamide adenine dinucleotide and returned to the electrochemical device for reduction.
Claims18
A system for electrochemically generating NAD(P)H2 reducing equivalents comprising:
- an electrochemical cell comprising an anode contained in an anode chamber and a cathode contained in a cathode chamber;
- a first process stream containing NAD(P), passing through the cathode chamber and continuously in contact with the cathode from which electrons are transferred to the NAD(P) to produce a second process stream containing reduced species 1,4-NAD(P)H2, 1,2-NAD(P)H2, 1,6-NAD(P)H2, and [NAD(P)]2, while optionally producing hydrogen;
- a substrate of an oxidoreductase or P450 enzyme, which, when contacted with the second process stream in the presence of the oxidoreductase or P450 enzyme, is transformed to a product while concomitantly consuming the 1,4-NAD(P)H2 in the second process stream and producing a first recovered NAD(P) and a third process stream;
- and
- at least one of a Mung Bean Phenol Oxidase enzyme and illumination at a wavelength of about 254 nm or exceeding about 320 nm, which, when contacted with the third process stream, convert at least one of the 1,2-NAD(P)H2, 1,6-NAD(P)H2, and [NAD(P)]2 therein to a second and optionally a third recovered NAD(P).
A method for electrochemically generating NAD(P)H2 reducing equivalents comprising:
- a. providing an electrochemical cell comprising an anode contained in an anode chamber and a cathode contained in a cathode chamber;
- b. passing through the cathode chamber a first process stream which contains NAD(P) and is continuously in contact with the cathode from which electrons are transferred to the NAD(P) to produce a second process stream containing reduced species 1,4-NAD(P)H2, 1,2-NAD(P)H2, 1,6-NAD(P)H2, and [NAD(P)]2, while optionally producing hydrogen;
- c. contacting the second process stream with a substrate of an oxidoreductase or P450 enzyme such that the substrate, in the presence of the oxidoreductase or P450 enzyme, is transformed to a product while concomitantly consuming the 1,4-NAD(P)H2 in the second process stream and producing a first recovered NAD(P) and a third process stream;
- and
- d. contacting the third process stream with at least one of a Mung Bean Phenol Oxidase enzyme and illumination at a wavelength of about 254 nm or exceeding about 320 nm, thereby converting at least one of the 1,2-NAD(P)H2, 1,6-NAD(P)H2, and [NAD(P)]2 therein to a second recovered NAD(P) and optionally a third recovered NAD(P).
Dependencies are inferred from the claim text.