Dna molecule coding for 5' utr enabling high recombinant protein expression in monocotyledons
AssigneeNational University Corporation Nara Institute of Science and Technology
InventorKo Kato
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Abstract
The purpose of the present invention is to provide technology for identifying a 5′ UTR having excellent translation ability while taking into account 5′ UTR variants in monocotyledons, providing a DNA molecule that codes for the 5′ UTR, and efficiently producing a recombinant protein in the monocotyledons. It is possible to efficiently produce a recombinant protein in a monocotyledon by using a nucleic acid structure in a polynucleotide that codes for a foreign protein, said structure comprising a base sequence among the base sequences shown in sequences 1-4 and linking to a 5′ UTR or a variant thereof.
Claims6
A DNA molecule encoding a 5′UTR, having:
- a polynucleotide shown in any of (i) to (iii) below:
- (i) a polynucleotide consisting of a base sequence shown in any of SEQ ID NOs:
- 1 to 4,
- (ii) a polynucleotide that consists of a base sequence modified from the base sequence shown in any of SEQ ID NOs:
- 1 to 4 by substitution, deletion, or addition of one or several bases, and exhibits 5′UTR activity equivalent to that of the polynucleotide consisting of the base sequence shown in any of SEQ ID NOs:
- 1 to 4, and
- (iii) a polynucleotide that hybridizes under a stringent condition with a DNA fragment consisting of a base sequence complementary to the base sequence shown in any of SEQ ID NOs:
- 1 to 4, and exhibits 5′UTR activity equivalent to that of the polynucleotide consisting of the base sequence shown in any of SEQ ID NOs:
- 1 to 4.
Dependencies are inferred from the claim text.