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US 2021/0171964 A1Published application
Plant transformation without selection
AssigneeMonsanto Technology LLC
InventorJyoti R. Rout
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Abstract
The invention provides methods for identifying regenerated transformed plants and differentiated transformed plant parts, obtained without subjecting plant cells to selective conditions prior to regenerating the cells to obtain differentiated tissues. In particular embodiments, the plant cells are corn plant cells. Methods for growing and handling plants, including identifying plants that demonstrate specific traits of interest are also provided.
Claims23
A method for identifying transgenic corn plants, comprising:
- (a) obtaining corn plant cells transformed with a DNA segment comprising a nucleic acid sequence of interest;
- (b) regenerating a plurality of corn plants or differentiated corn plant parts from the cells without first selecting for the presence of said DNA segment;
- (c) identifying at least a first transgenic corn plant or transgenic differentiated plant part from the plurality of corn plants or differentiated corn plant parts.
Dependencies are inferred from the claim text.