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US 2021/0171673 A1Published application
Polymer-Encased Nanodiscs With Improved Buffer Compatibility
AssigneeTexas Tech University System
InventorsGuillermo A. Altenberg, Hongjun Liang
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Abstract
The present invention includes compositions, methods, and methods of making and using a polymer-encased nanodisc comprising: one or more integral membrane proteins in a lipid layer; and a polymer comprising zwitterionic styrene-maleic acid derivative repeating units that carry zero or nearly zero negative charge, and the polymer-encased nanodiscs.
Claims23
Polymer-encased nanodiscs with improved buffer compatibility comprising:
- a polymer comprising a styrene-maleic acid derivative repeating units that carries zero or nearly zero net negative charge:
- wherein the distribution of styrene and maleic acid derivative repeating units is random, and the resultant nanodiscs are compatible with at least one of polyvalent cations, MgCl2, CaCl2, or pH<5, wherein R1, R2, and R3, are selected from hydrogen;
- deuterium;
- a halogen group;
- a nitrile group;
- a nitro group;
- an imide group;
- a hydroxyl group;
- a substituted or unsubstituted alkyl group;
- a substituted or unsubstituted cycloalkyl group;
- a substituted or unsubstituted alkoxy group;
- a substituted or unsubstituted aryloxy group;
- a substituted or unsubstituted alkylthioxy group;
- a substituted or unsubstituted arylthioxy group;
- a substituted or unsubstituted alkylsulfoxy group;
- a substituted or unsubstituted arylsulfoxy group;
- a substituted or unsubstituted alkenyl group;
- a substituted or unsubstituted silyl group;
- a substituted or unsubstituted boron group;
- a substituted or unsubstituted alkylamine group;
- a substituted or unsubstituted aralkylamine group;
- a substituted or unsubstituted arylamine group;
- a substituted or unsubstituted heteroarylamine group;
- a substituted or unsubstituted aryl group;
- or a substituted or unsubstituted heterocyclic group including one or more of N, O, or S atoms;
- and wherein n is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160, 165, 170, 175, 185, 190, 195, 200, or more.
23 more elements
A method of making a nanodisc with improved buffer compatibility comprising:
- mixing the one or more integral membrane proteins in a lipid layer with a polymer comprising a styrene-maleic acid derivative repeating units that carries zero or nearly zero net negative charge:
- wherein the distribution of styrene and maleic acid derivative repeating units is random, wherein the nanodiscs are compatible with at least one of polyvalent cations, MgCl2, CaCl2, or pH<5, wherein R1, R2, R3, and R4 are selected from hydrogen;
- deuterium;
- a halogen group;
- a nitrile group;
- a nitro group;
- an imide group;
- a hydroxyl group;
- a substituted or unsubstituted alkyl group;
- a substituted or unsubstituted cycloalkyl group;
- a substituted or unsubstituted alkoxy group;
- a substituted or unsubstituted aryloxy group;
- a substituted or unsubstituted alkylthioxy group;
- a substituted or unsubstituted arylthioxy group;
- a substituted or unsubstituted alkylsulfoxy group;
- a substituted or unsubstituted arylsulfoxy group;
- a substituted or unsubstituted alkenyl group;
- a substituted or unsubstituted silyl group;
- a substituted or unsubstituted boron group;
- a substituted or unsubstituted alkylamine group;
- a substituted or unsubstituted aralkylamine group;
- a substituted or unsubstituted arylamine group;
- a substituted or unsubstituted heteroarylamine group;
- a substituted or unsubstituted aryl group;
- or a substituted or unsubstituted heterocyclic group including one or more of N, O, or S atoms;
- and wherein n is 1 to 200.
23 more elements
A method of making a nanodisc with improved buffer compatibility comprising:
- mixing a cell membrane carrying integral membrane proteins with a polymer comprising zwitterionic styrene-maleic acid (zSMA) repeating units:
- wherein the distribution of styrene and maleic acid derivative repeating units is random, and the zSMA is compatible with at least one of polyvalent cations, MgCl2, CaCl2, or pH<5, wherein R5, R6, R7, R8, R9, R10, R11, R12, R13 are selected from hydrogen;
- deuterium;
- a halogen group;
- a nitrile group;
- a nitro group;
- an imide group;
- a hydroxyl group;
- a substituted or unsubstituted alkyl group;
- a substituted or unsubstituted cycloalkyl group;
- a substituted or unsubstituted alkoxy group;
- a substituted or unsubstituted aryloxy group;
- a substituted or unsubstituted alkylthioxy group;
- a substituted or unsubstituted arylthioxy group;
- a substituted or unsubstituted alkylsulfoxy group;
- a substituted or unsubstituted arylsulfoxy group;
- a substituted or unsubstituted alkenyl group;
- a substituted or unsubstituted silyl group;
- a substituted or unsubstituted boron group;
- a substituted or unsubstituted alkylamine group;
- a substituted or unsubstituted aralkylamine group;
- a substituted or unsubstituted arylamine group;
- a substituted or unsubstituted heteroarylamine group;
- a substituted or unsubstituted aryl group;
- or a substituted or unsubstituted heterocyclic group including one or more of N, O, or S atoms;
- and
- wherein n is 1 to 200, or more.
24 more elements
A composition comprising:
- wherein R5, R6, R7, R8, R9, R10, R11, R12, R13 are selected from hydrogen;
- deuterium;
- a halogen group;
- a nitrile group;
- a nitro group;
- an imide group;
- a hydroxyl group;
- a substituted or unsubstituted alkyl group;
- a substituted or unsubstituted cycloalkyl group;
- a substituted or unsubstituted alkoxy group;
- a substituted or unsubstituted aryloxy group;
- a substituted or unsubstituted alkylthioxy group;
- a substituted or unsubstituted arylthioxy group;
- a substituted or unsubstituted alkylsulfoxy group;
- a substituted or unsubstituted arylsulfoxy group;
- a substituted or unsubstituted alkenyl group;
- a substituted or unsubstituted silyl group;
- a substituted or unsubstituted boron group;
- a substituted or unsubstituted alkylamine group;
- a substituted or unsubstituted aralkylamine group;
- a substituted or unsubstituted arylamine group;
- a substituted or unsubstituted heteroarylamine group;
- a substituted or unsubstituted aryl group;
- or a substituted or unsubstituted heterocyclic group including one or more of N, O, or S atoms;
- and wherein n is 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, 105, 110, 115, 120, 125, 130, 135, 140, 145, 150, 155, 160, 165, 170, 175, 185, 190, 195, 200, or more.
22 more elements
A composition made by a method comprising:
Dependencies are inferred from the claim text.