Cell-free protein synthesis platform derived from cellular extracts of vibrio natriegens
AssigneeNorthwestern University
InventorsMichael Christopher Jewett, Benjamin James Des Soye, Samuel Ryan Davidson
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Abstract
Disclosed are compositions, methods, and kits for performing cell-free RNA transcription and/or cell-free protein synthesis (CFPS). The disclosed compositions, methods, and kits include or utilize components prepared from Vibrio species such as cellular extracts from Vibrio natriegens.
Claims24
A platform for in vitro transcription of mRNA and/or translation of peptides, polypeptides, or sequence defined polymers, the platform comprising a cellular extract prepared from a cell culture of a species of Vibrio.
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A kit comprising as components:
- (a) a cellular extract prepared from a cell culture of a species of Vibrio;
- and (b) a reaction mixture for translating an mRNA, optionally wherein the species of Vibrio is Vibrio natriegens.
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A recombinant species of Vibrio, optionally Vibrio natriegens, wherein the species of Vibrio is engineered to be deficient in a negative effector for cell-free protein synthesis (CFPS).
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Dependencies are inferred from the claim text.