Improved protein recovery
AssigneeBristol-Myers Squibb Company
InventorsAbhiram Arunkumar, Nripen Singh, Ameya Umesh Borwankar
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Abstract
This disclosure provides a novel flush method using a recovery flush technique that minimizes yield losses due to inadequate flushing and prevents or reduces dilution of a target protein during a recovery flush.
Claims50
A method of reducing or preventing dilution of a target protein during a recovery flush after a filtration comprising (i) passing a sample containing the target protein through a filtration assembly at a feed flow rate while the filtration is on and (ii) passing a buffer through the filtration assembly during a recovery flush at a flush flow rate that is less than 100 liters per square meter per hour (LMH), thereby reducing or preventing dilution of the target protein compared to the dilution of a target protein obtained with a flush flow rate of 300 LMH.
A method of improving or increasing a concentration of a target protein during a recovery flush after a filtration comprising (i) passing a sample containing the target protein through a filtration assembly at a feed flow rate while the filtration is on and (ii) passing a flush buffer through the filtration assembly during a recovery flush at a flush flow rate that is less than 100 liters per square meter per hour (LMH), thereby improving the target protein concentration compared to a target protein concentration obtained with a flush flow rate of 300 LMH.
The method of any one of claims 1 to 17, wherein the yield of the target protein after the recovery flush is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or at least 100% compared to the concentration of the target protein prior to the recovery flush process consisting of (i) and (ii).
Dependencies are inferred from the claim text.