Method for analysis of chondroitin sulfate
AssigneeJCR Pharmaceuticals Co., Ltd.
InventorsNoboru TANAKA, Sachiho KIDA
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Abstract
Disclosed is a method for decomposing chondroitin sulfate contained in a sample into disaccharide. In particular disclosed is a method for decomposing chondroitin sulfate contained in a sample into disaccharide by heating the chondroitin sulfate in HCl-methanol containing 2,2-dimethoxypropane at a temperature of 60° C. to 90° C. for 50 minutes to 180 minutes, optionally in the method, the sample is selected from body fluid, a cell, a tissue, an organ, a cell culture solution, a tissue culture solution, a food, and a feed, or a derived therefrom.
Claims26
A method for decomposing chondroitin sulfate contained in a sample into disaccharide represented by formula (IV) below:
- wherein the chondroitin sulfate is decomposed by heating in HCl-methanol containing 2,2-dimethoxypropane at a temperature of 60° C. to 90° C. for 50 minutes to 180 minutes.
Dependencies are inferred from the claim text.