The method(s) of bioremediation of crude oil spills in sea water in the presence of other carbon sources, wherein the method enables instant breakdown of crude oil spills, the method comprising:
- Using a system to contain the crude oil at the surface of sea with minimal amount of sea water within this system,
- Using the composition in a particular method to enable instant bioremediation in sea water, the proportions could be:
- 100 ml sea water to which 50 drops of crude oil has been added. To this 100 ml sea water with 50 drops of crude oil, (10-50) ml of growth media is added, for example Nutrient Broth is added. Then, the bacterial suspensions are added in the proportions ranging from (0.5:0.5:2) to (1:1:3) in various permutations and combinations for (New species belonging to family Pseudomonadaceae and showing 75%-85% similarity to genus Pseudomonas was isolated and used in the composition). The bacterial cultures were introduced sequentially, either as suspension cultures or as cell mass. This formulation is then mixed well. The custom designed system(s) enables mixing to ensure complete bioremediation of crude oil spills in sea water.
- The bioremediated water is passed through the biofiltration system before entering the sea water.