Salt formulations for the fermentation of marine microorganisms
AssigneeCelgene International II SÀRL
InventorsKin Sing LAM, Ginger TSUENG
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Abstract
Growth medium are disclosed for use in fermenting a marine microorganism. The medium comprise Potassium, Calcium, Strontium, Borate and Fluoride at specific concentrations. Alternatively, the growth medium comprises cobalt at specified concentrations or comprises vitamin B12 at specified concentrations. Methods of producing certain desired compound by fermentation of a marine microorganism are also disclosed.
Claims25
-29. (canceled)
A method of producing Salinosporamide A, comprising the steps of
- (i) preparing a microbial growth medium;
- (ii) inoculating said microbial growth medium with a strain of microorganism capable of producing Salinosporamide A;
- (iii) adding a resin to the microbial growth medium before 96 hours after inoculation of the media,
- wherein the growth medium comprises CaCO3, Fe2(SO4)3, and KBr, and wherein the final concentration of the resin in the growth medium is 20 g/L, 30 g/L, or 40 g/L.
(canceled)
(canceled)
-48. (canceled)
A method of producing a compound of Formula A;
- wherein R1 is selected from the group consisting of hydrogen and hydroxide, R2 is selected from the group consisting of 2-chloroethyl, 2-bromoethyl, ethyl, methyl, and propyl, and R3 is selected from the group consisting of methyl and ethyl, comprising the steps of growing a culture of S. tropica in the presence of XAD-7 resin.
-59. (canceled)
A salt formulation for use in fermenting a marine microorganism, the formulation comprising:
- KCl at a mass percent between 50% and 70%;
- CaCl2 at a mass percent between 30% and 45%;
- SrCl2 at a mass percent between 1% and 3%;
- H3BO3 at a mass percent between 1% and 3%;
- and
- NaF at a mass percent between 0.1% and 0.3%.
Dependencies are inferred from the claim text.