A method to homogenize parvovirus b19 in clinical samples
AssigneeGrifols Diagnostic Solutions Inc.
InventorsKui Gao, Jeffrey M. Linnen
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Abstract
Various aspects of the invention relate to compositions and methods of analyzing blood plasma, blood serum, and manufacturing pools of plasma-derived products wherein an anionic surfactant is added to an aliquot of the blood plasma, blood serum, or manufacturing pool prior to analysis, and the counterion of the anionic surfactant is not sodium ion. The anionic surfactant may be, for example, lauryl sulfate. The counterion of the anionic surfactant may be, for example, lithium ion.
Claims32
A method of determining the concentration of human parvovirus B19 in human blood plasma or a manufacturing pool of a human plasma-derived product, comprising:
- contacting a sample with a surfactant solution comprising an anionic surfactant thereby producing a treated sample, wherein the sample comprises an aliquot of either human blood plasma or a manufacturing pool of a human plasma-derived product;
- and
- determining whether the concentration of human parvovirus B19 in the aliquot is above or below a threshold value,
- wherein:
- the molar ratio of sodium to anionic surfactant in the surfactant solution is less than 1:10.
A method of manufacturing a plasma-derived product, comprising:
- performing the method of any one of the preceding embodiments;
- and
- manufacturing a plasma-derived product from the human blood plasma or the manufacturing pool if the concentration of human parvovirus B19 in the aliquot is less than the threshold value.
A composition comprising:
- a Parvoviridae virus genome;
- an anionic surfactant;
- and
- at least two amplification primers that each specifically bind either a nucleotide sequence of a Parvoviridae virus genome or a reverse complement thereof thereby allowing the amplification of a Parvoviridae virus nucleotide sequence,
- wherein:
- the composition comprises a liquid;
- the liquid comprises the Parvoviridae virus genome and the anionic surfactant;
- and
- the molar ratio of the anionic surfactant to any sodium ion present in the liquid is greater than 1:1.
Dependencies are inferred from the claim text.