Derivation of human skin organoids from pluripotent stem cells
AssigneeIndiana University Research and Technology Corporation
InventorsKarl Russell Koehler, Jiyoon Lee
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Abstract
Provided herein are methods for directing differentiation of human pluripotent stem cells into a three-dimensional multilayered skin composition comprising an epidermal layer, a dermal layer, and a plurality of cells capable of forming a functional hair follicle. Also provided herein are three-dimensional, multilayered engineered skin compositions and methods of using the same for drug screening, for screening compounds for effects on hair growth, and for other applications.
Claims12
-12. (canceled)
A three-dimensional, multilayered engineered skin composition comprising an epidermal layer comprising human pluripotent stem cell-derived epidermal keratinocytes, a dermal layer comprising human pluripotent stem cell-derived dermal fibroblasts, and a plurality of human pluripotent stem cell-derived cells capable of forming a functional hair follicle, wherein the epidermal layer and dermal layer are in direct contact.
A method of testing a compound for effects on hair growth, the method comprising
- (a) contacting a test compound to a three-dimensional multilayered skin composition obtained according to the method of claim 1;
- and
- (b) detecting an effect of the agent on one or more cell types within the contacted skin composition.
Use of a three-dimensional multilayered skin composition obtained according to the method of claim 1 in a drug discovery screen.
Dependencies are inferred from the claim text.