Method of isolating and detecting cancer stem cells

Method for in vitro diagnosis of the risk of recurrence of cancer of an organ involved in respiration and/or of the aggressiveness of cancer of an organ involved in respiration to define a prognostic value for the therapeutic adaptation of a cancer of an organ involved in respiration, comprising a step of labelling the cancer stem cells of organs involved in the respiration of a biological sample of organs involved in respiration with at least one lectin recognizing the fucose α 1-2 galactose unit, to obtain cancer stem cells of organs involved in respiration labelled by at least one lectin in said sample.

An in vitro kit for the diagnosis of the risk of recurrence of cancer of an organ involved in respiration and/or of the aggressiveness of cancer of an organ involved in respiration to define a prognostic value for the therapeutic adaptation of a cancer of an organ involved in respiration,

• comprising at least one lectin recognizing the fucose α 1-2 galactose group, said lectin being conjugated to biotin, and magnetic beads functionalized with streptavidin, and optionally at least one lectin recognizing the fucose α 1-2 galactose group, conjugated to a fluorophore, a radioisotope, an enzyme or gold beads, or
• comprising at least one lectin recognizing the fucose α 1-2 galactose group, said lectin being conjugated a fluorophore, and optionally at least one lectin recognizing the fucose α 1-2 galactose group, conjugated to biotin, a radioisotope, an enzyme or gold beads,
• in particular wherein said fucose α 1-2 galactose group is the fucose α 1-2 galactose β 1-4 N-acetylglucosamine group.