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US 2021/0079463 A1Published application
Methods and systems for processing polynucleotides
Assignee10x Genomics, Inc.
InventorsBenjamin Hindson, Serge Saxonov, Kevin Ness, Paul Hardenbol, Michael Schnall-Levin, Mirna Jarosz
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Abstract
The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.
Claims21
A method comprising:
- a. providing a target polynucleotide;
- b. fragmenting said target polynucleotide to generate a plurality of non-overlapping first polynucleotide fragments;
- c. partitioning said first polynucleotide fragments to generate partitioned first polynucleotide fragments, wherein at least one partition of said partitioned first polynucleotide fragments comprises a first polynucleotide fragment with a unique sequence within said at least one partition;
- and
- d. fragmenting said partitioned first polynucleotide fragments to generate a plurality of non-overlapping second polynucleotide fragments.
2 more elements
-67. (canceled)
Dependencies are inferred from the claim text.