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AssigneeKeygene N.V.
InventorsMichiel Theodoor Jan DE BOTH, Rik Hubertus Martinus OP DEN CAMP, Bjorn Alexander KLOOSTERMAN, Bernarda Gerharda Johanna FIERENS-ONSTENK
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Genetic modification of plants is hampered by the limited capacity of plant cells to regenerate. The current invention solves this problem by introducing or increasing the expression of a histidine kinase in a plant cell. Preferred histidine kinases are at least one of CHK2, CHK3 and CHK4. The invention therefore concerns a method for improving a cytokinin-induced regeneration capacity of a plant cell, wherein the method comprises a step of increasing or introducing the expression of a histidine kinase in the plant cell. The invention further pertains to a method for regenerating a plant, wherein the method comprises a step of introducing or increasing the expression of a histidine kinase and to a plant obtainable from such method. Moreover, the method concerns the use of at least one of CHK2, CHK3 and CHK4 for improving a cytokinin-induced regeneration capacity of a plant.
A method for improving cytokinin-induced regeneration capacity of a plant cell, comprising increasing or introducing expression of at least one histidine kinase in the plant cell, wherein the histidine kinase is selected from the group consisting CHK4, CHK2 and CHK3.
A method for regenerating a plant, comprising:
An expression construct comprising a first nucleotide sequence having at least 50% sequence identity with SEQ ID NO:
Dependencies are inferred from the claim text.