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US 2021/0079413 A1Published application
Methods and materials for high throughput testing of mutagenized allele combinations
AssigneeCeres, Inc.
InventorsRoger I. Pennell, Richard Hamilton, Delin Liang
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Abstract
High throughput methods are described for identifying combinations of mutations that can be used to improve a phenotypic feature in an organism. Large populations of organisms (e.g., plants) containing different combinations of mutations can be assessed using the methods.
Claims24
A method for identifying a combination of genetic mutations that improves a phenotype of a plant, wherein said method comprises:
- (a) selecting a plurality of genomic targets for mutation,
- (b) making a plant cell comprising a plurality of different gRNAs designed to mutate the genomic targets, and a Cas9 polypeptide, wherein a plant descended from the plant cell has a plurality of germline mutations,
- (c) sexually crossing a first parental plant comprising at least a subset of the germline mutations to a second parental plant to produce a progeny population,
- (d) phenotyping the progeny population to select an individual with improved phenotype, and
- (e) genotyping the selected individual to identify the combination of genetic mutations that improves the phenotype of the plant.
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