Engineering wheat with increased dietary fiber
AssigneeCellectis
InventorsNicholas BALTES, Javier GIL HUMANES
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Abstract
Materials and methods are provided for making plants (e.g., Triticum varieties) with increased levels of dietary fiber, such as by making TALE nuclease-induced mutations in alleles encoding starch branching enzyme IIa (SBEIIa) and starch branching enzyme IIb (SBEIIb).
Claims35
A Triticum plant, plant part, or plant cell comprising a deletion or insertion in at least two starch branching enzyme II (SBEII) alleles endogenous to the plant, plant part, or plant cell, wherein the deletion or insertion was made using a rare-cutting endonuclease.
A method for generating a Triticum plant that has increased levels of dietary fiber, wherein the method comprises:
- (a) contacting a Triticum plant cell or plant part comprising functional SBEII alleles with a rare-cutting endonuclease targeted to an endogenous SBEII allele;
- (b) selecting from the plant cell or plant part a plant cell or plant part in which at least one SBEII allele comprises a deletion or insertion due, at least in part, to nuclease activity of the rare-cutting endonuclease;
- and
- (c) growing the selected plant cell or plant part into a Triticum plant, wherein the Triticum plant has increased levels of dietary fiber as compared to a control Triticum plant in which the SBEII alleles have not been mutated.
Dependencies are inferred from the claim text.