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US 2021/0079355 A1Published application
Sc-beta cells and compositions and methods for generating the same
AssigneePresident and Fellows of Harvard College
InventorsQuinn P. Peterson, Felicia J. Pagliuca, Douglas A. Melton, Jeffrey R. Millman, Michael Saris Segel, Mads Gurtler
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Abstract
Disclosed herein are methods, compositions, kits, and agents useful for inducing β cell maturation, and isolated populations of SC-β cells for use in various applications, such as cell therapy.
Claims28
An in vitro composition that comprises a TGF-beta superfamily growth factor, a sonic hedgehog pathway inhibitor, and a plurality of non-native human pancreatic progenitor cells that express PDX1.
(canceled)
A method of in vitro cell differentiation, comprising:
- contacting an in vitro cell population comprising pancreatic progenitor cells that express PDX1 but not NKX6.1 with a TGF-beta superfamily growth factor, an FGF family growth factor, a sonic hedgehog pathway inhibitor, and a retinoic acid signaling pathway activator,
- wherein the method results in a plurality of the pancreatic progenitor cells expressing PDX1 but not NKX6.1 in the population to differentiate into pancreatic progenitor cells that express PDX1 and NKX6.1.
Dependencies are inferred from the claim text.