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US 2021/0069255 A1Published application
Methods for obtaining muscle derived cells
AssigneeInnovacell AG
InventorsMarco THURNER, Eva MARGREITER, Wolfgang SCHWAIGER, Faheem Muhammad ASIM, Rainer MARKSTEINER
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Abstract
The present invention relates to methods for obtaining skeletal muscle derived cells (SMDC), and the use of SMDCs in a method of preventing and/or treating neuromyopathies and/or myopathies, wherein the neuromyopathy and/or myopathy is incontinence, in particular a urinary and/or an anal or fecal incontinence.
Claims19
A method for obtaining skeletal muscle derived cells (SMDCs), the method comprises the steps of:
- (a) cooling of a sample obtained from skeletal muscle tissue in a buffer;
- (b) processing and cooling of the sample;
- (c) resuspending the sample of step (b) in medium with serum comprising at least one enzyme and heating up to 38° C. for 1 to 20 hours;
- pelleting the sample and
- (d) resuspending the pellet of the sample of step (c) to provide a single cell suspension from the sample of step (c), thereby obtaining SMDCs.
2 more elements
A skeletal muscle derived cell (SMDC) population, wherein the population comprises at least 60% CD56 positive and 60% A2B5 positive cells.
Dependencies are inferred from the claim text.