Fluorescent image analyzer

A fluorescent image analyzer comprising:

• one or more processors;
• and
• one or more storage devices,
• wherein the one or more storage devices store a reference fluorescent-sample image and a subject fluorescent-sample image,
• wherein the reference fluorescent-sample image is an image obtained by illuminating a reference fluorescent sample about which relation of in-plane fluorescence intensities is known with linearly polarized light and capturing a first predetermined polarization component of fluorescence from the reference fluorescent sample,
• wherein the subject fluorescent-sample image is an image obtained by illuminating a subject fluorescent sample with linearly polarized light and capturing a second predetermined polarization component of fluorescence from the subject fluorescent sample, and
• wherein the one or more processors are configured to:
• determine correction coefficients to correct non-uniformity in measurement of light intensities among pixels of a captured image based on the reference fluorescent-sample image;
• and
• correct light intensities of the pixels of the subject fluorescent-sample image based on the correction coefficients.