Method for producing library for use in prediction of action of substance of interest on living body, and method for evaluating action of substance of interest on living body based on change in action parameter or the like of zebrafish
AssigneesSuntory Holdings Limited, Mie University
InventorsMikiko YAMANAKA, Toshio TANAKA, Junko KOIWA
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Abstract
A method for producing a library for use in prediction of the action of a substance of interest on a living organism, the library having a large number of numerical data obtained based on change in a behavioral parameter of zebrafish and a large number of numerical data representing change in expression levels of genes integrated and recorded therein.
Claims10
A method for producing a library for use in prediction of the action of a substance of interest on the living organism, the library having a large number of numerical data obtained based on change in a behavioral parameter of zebrafish and a large number of numerical data representing change in expression levels of genes integrated and recorded therein, the method comprising:
- preparing a substance of known action that is a substance whose action on the living organism is known;
- measuring a behavioral parameter of zebrafish not given the substance of known action in a bath having the zebrafish therein;
- administering the substance of known action to the bath having the zebrafish therein and measuring the behavioral parameter of the zebrafish after the administration of the substance of known action in the bath having the zebrafish therein;
- detecting change in the behavioral parameter of the zebrafish by the administration of the substance of known action;
- subdividing data representing the change in the obtained behavioral parameter and converting the data into a large number of numerical data;
- normalizing the large number of numerical data obtained based on the change in the behavioral parameter;
- normalizing a large number of numerical data representing the change in expression levels of genes after the administration of the substance of known action;
- and
- integrating and recording the large number of numerical data obtained based on the change in the behavioral parameter and the large number of numerical data representing change in expression levels of genes, both normalized.
A method for evaluating the action of a substance of interest on the living organism, comprising:
- preparing a library having a large number of numerical data obtained based on change in a behavioral parameter of zebrafish and a large number of numerical data representing change in expression levels of genes, both normalized, integrated, and recorded therein;
- measuring a behavioral parameter of zebrafish not given a substance of interest in a bath having the zebrafish therein;
- administering the substance of interest to the bath having the zebrafish therein and measuring the behavioral parameter of the zebrafish after the administration of the substance of interest in the bath having the zebrafish therein;
- normalizing a large number of numerical data obtained based on change in the behavioral parameter of zebrafish by the administration of the substance of interest;
- identifying a gene whose expression level is changed in correlation with the administration of the substance of interest by statistically matching and analyzing the large number of numerical data obtained based on change in the behavioral parameter of zebrafish by the administration of the substance of interest and the large number of numerical data obtained based on change in the behavioral parameter of zebrafish and the large number of numerical data representing change in expression levels of genes integrated and recorded in the library, both normalized;
- thereby predicting action of the substance of interest on the living organism based on function of the identified gene.
Dependencies are inferred from the claim text.