Compositions and methods for identifying a single-nucleotide variant

A method of measuring the rate of accumulation of genome-wide somatic single-nucleotide variants (SNVs), the method comprising:

• identifying double-stranded mutations in a genomic sequence derived from a biological sample, wherein the double-stranded mutations comprise a variant nucleotide on forward and reverse strands;
• and
• performing linkage analysis to obtain a frequency of observed double-stranded mutations adjusted for the number of regions linked to a germline heterozygous variant, thereby measuring the rate of accumulation of genome-wide somatic SNVs.

A method of measuring the somatic mutation burden of a subject, the method comprising:

• identifying double-stranded mutations in a genomic sequence from a biological sample from the subject, wherein the double-stranded mutations comprise a variant nucleotide on forward and reverse strands;
• and
• performing linkage analysis to obtain a frequency of observed double-stranded mutations adjusted for the number of regions linked to a germline heterozygous variant, thereby measuring the somatic mutation burden in the subject.