Methods for ethanol production using engineered yeast
AssigneeCargill, Incorporated
InventorsGregory M. Poynter, Brian J. Rush, Sneha Srikrishnan, Dawn Thompson, Arthur Shockley, Brynne Kohman, Joshua Dunn
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Abstract
Aspects of the disclosure provide engineered microbes for ethanol production. Methods for microbe engineering and culturing are also provided herein. Such engineered microbes exhibit enhanced capabilities for ethanol production.
Claims47
An engineered yeast comprising:
- a recombinant nucleic acid encoding a glyceraldehyde-3-phosphate dehydrogenase (E.C. 1.2.1.9);
- reduced or eliminated expression of a gene encoding a glycerol-3-phosphate phosphatase (E.C. 3.1.3.21);
- and a recombinant nucleic acid encoding a glucoamylase, wherein the yeast is capable of producing at least 100 g/kg of ethanol and producing less than 1.5 g/kg residual glucose in 48 hours under Test 1 conditions.
An engineered Saccharomyces cerevisiae yeast comprising:
- a recombinant nucleic acid encoding a glyceraldehyde-3-phosphate dehydrogenase (E.C. 1.2.1.9);
- and reduced or eliminated expression of a gene encoding a glycerol-3-phosphate phosphatase (E.C. 3.1.3.21), wherein the yeast is capable of producing at least 100 g/kg of ethanol and producing less than 1.5 g/kg residual glucose in 48 hours under Test 2 conditions.
An engineered yeast comprising an exogenous nucleic acid encoding a glyceraldehyde-3-phosphate dehydrogenase (E.C. 1.2.1.9), and an exogenous nucleic acid encoding a glucoamylase (GA) having 80% or greater identity to SEQ ID NO:38 (Saccharomycopsis fibuligera GA), SEQ ID NO:41 (Rhizopus microsporus GA), SEQ ID NO:40 (Rhizopus delemar GA), or SEQ ID NO:39 (Rhizopus oryzae amyA), wherein the yeast is capable of producing at least 100 g/kg of ethanol and having less than 1.5 g/kg residual glucose in 48 hours under Test 1 conditions.
Dependencies are inferred from the claim text.