ATF5 Peptide Variants and Uses Thereof
AssigneeSapience Therapeutics, Inc.
InventorsBarry Jay Kappel, Gene Merutka, Jimmy Andrew Rotolo
Life of this patent
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Abstract
Provided are ATF5 peptides having a truncated ATF5 leucine zipper region and, optionally, a cell-penetrating region, compositions comprising the ATF5 peptides, and methods of inhibiting proliferation of and promoting cytotoxicity in a neoplastic cell using the ATF5 peptides.
Claims18
An ATF5 peptide comprising a truncated ATF5 leucine zipper region, wherein the truncated ATF5 leucine zipper region comprises a variant of the amino acid sequence LEGECQGLEARNRELKERAESV (SEQ ID NO:
- 53), wherein the variant is modified at one or more positions of SEQ ID NO:
- 53 as follows:
- i. E4 is substituted with arginine;
- ii. C5 is substituted with alanine or glycine;
- iii. Q6 is substituted with alanine;
- iv. E9 is substituted with arginine;
- v. R11 is substituted with glutamic acid;
- vi. N12 is substituted with leucine;
- vii. K16 is substituted with glutamic acid;
- viii. S21 is substituted with alanine.
An ATF5 peptide comprising a truncated ATF5 leucine zipper region, wherein the truncated ATF5 leucine zipper region comprises an amino acid sequence selected from the group consisting of:
- LEGEGQGLEARNRELKERAESV (SEQ ID NO:
- 54), LEGEAQGLEARNRELKERAESV (SEQ ID NO:
- 55), LEGECQGLEARNRELKERAEAV (SEQ ID NO:
- 56), LEGECQGLEARLRELKERAESV (SEQ ID NO:
- 57), LEGECAGLEARNRELKERAESV (SEQ ID NO:
- 58), LEGRCQGLRAENRELEERAESV (SEQ ID NO:
- 59), LEGRCQGLRAELRELEERAEAV (SEQ ID NO:
- 60), and LEGRAQGLRAELRELEERAEAV (SEQ ID NO:
- 61).
A cell-penetrating ATF5 peptide comprising a variant of the amino acid sequence RQIKIWFQNRRMKWKKLEGECQGLEARNRELKERAESV (SEQ ID NO:
- 3), wherein the variant is modified at positions of SEQ ID NO:
- 3 selected from the group consisting of:
- i. C21G (SEQ ID NO:
- 4);
- ii. C21A (SEQ ID NO:
- 5);
- iii. Q22A (SEQ ID NO:
- 8);
- iv. E20R, E25R, R27E, and K32E (SEQ ID NO:
- 9);
- v. N28L (SEQ ID NO:
- 7);
- vi. S37A (SEQ ID NO:
- 6);
- vii. E20R, E25R, R27E, N28L, K32E, and S37A (SEQ ID NO:
- 10);
- and
- viii. E20R, C21A, E25R, R27E, N28L, K32E, and S37A (SEQ ID NO:
- 11).
(canceled)
(canceled)
Dependencies are inferred from the claim text.