Dna gridiron compositions and methods
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsDongran HAN, Hao YAN
Life of this patent
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Abstract
Novel compositions and methods for engineering wireframe architectures and scaffolds of increasing complexity by creating gridiron-like DNA structures (FIG. 1). A series of four-arm junctions are used as vertices within a network of double-helical DNA fragments. Deliberate distortion of the junctions from their most relaxed conformations ensures that a scaffold strand can traverse through individual vertices in multiple directions. DNA gridirons, ranging from two-dimensional arrays with reconfigurability to multilayer and three-dimensional structures and curved objects, can be assembled according the methods presented herein.
Claims11
1-9. (canceled)
A composition comprising a plurality of immobile Holliday junction analogs linked together in a plurality of layered frames, wherein each layer of frame has at least two DNA helices which lie on opposite sides of the Holliday junction which also lie in the same plane, and wherein said plurality of immobile Holliday junction analogs are linked together with a central strand of single-stranded DNA within said layer of frame.
A composition comprising
- a plurality of immobile Holliday junction analogs linked together to form a structure selected from:
- (a) an S-shaped structure as depicted in FIG. 4A,
- (b) a spherical shaped structure as depicted in FIG. 4B, or
- (c) a corkscrew-shaped structure as depicted in FIG. 4C.
Dependencies are inferred from the claim text.