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US 2021/0060182 A1Published application
Rna transcription vector and uses thereof
AssigneeVrije Universiteit Brussel
InventorsCarlo Heirman, Kristiaan Thielemans
Life of this patent
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Abstract
The present invention in general relates to an improved RNA transcription vector, which is very suitable for the production of mRNA for in vivo therapeutic purposes. The improvements in the vector in particular reside in the presence of a transcription enhancer and a nuclear retention element.
Claims19
1-16. (canceled)
An RNA production vector comprising:
- a transcribable nucleic acid sequence;
- a 5′ translation enhancer (TE) sequence comprising a tandem repeat of a 9-nucleotide segment of the mouse Gtx homeodomain protein, interspaced by 9-nucleotide fragments of the human beta globin 5′ UTR;
- and
- a 3′ nuclear retention sequence (ENE) comprising the poly-adenylated non-translated RNA (PAN) region of the Kaposi's sarcoma associated Herpes virus (KSHV).
1 more element
An RNA molecule comprising:
- a transcribable nucleic acid sequence;
- a 5′ translation enhancer (TE) sequence comprising a tandem repeat of a 9-nucleotide segment of the mouse Gtx homeodomain protein, interspaced by 9-nucleotide fragments of the human beta globin 5′ UTR;
- and
- a 3′ nuclear retention sequence (ENE) comprising the poly-adenylated non-translated RNA (PAN) region of the Kaposi's sarcoma associated Herpes virus (KSHV).
1 more element
Dependencies are inferred from the claim text.