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US 2021/0011014 A1Published application
Detection and assay devices and methods of making and using the same
AssigneeSentilus Holdco LLC
InventorsAshutosh CHILKOTI, Angus HUCKNALL
Life of this patent
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Abstract
An article such as a biomolecular detector or biosensor having a nonfouling surface thereon includes:(a) a substrate having a surface portion; (b) a linking layer on the surface portion; and (c) a polymer layer formed on the linking layer; and (d) a first member of a specific binding pair (e.g., a protein, peptide, antibody, nucleic acid, etc.) bound to the polymer layer. Methods of making and using the articles are also described.
Claims16
1-35. (canceled)
A method of detecting the presence of an analyte in a sample suspected of containing the analyte, the method comprising
- (a) contacting the sample suspected of containing the analyte to a biomolecular detector, wherein the biomolecular detector comprises:
- (i) a substrate having a surface portion,
- (ii) a linking layer on the surface portion,
- (iii) a polymer layer formed on the linking layer, wherein the polymer comprises monomeric units, with each of the monomeric units comprising a monomer core group having at least one protein-resistant head group coupled thereto, to thereby form a brush molecule on the surface portion;
- wherein the brush molecule comprises a stem formed from the polymerization of the monomer core groups, and a plurality of branches formed from the head group projecting from the stem, and
- (iv) a probe directly non-covalently bound to the polymer layer;
- (b) incubating the sample on surface portion of the biomolecular detector to permit specific binding between the probe and the analyte, and
- (c) detecting the binding of the analyte to the probe, wherein detection of the binding of the analyte to the probe indicates the presence of the analyte in the sample.
5 more elements
Dependencies are inferred from the claim text.