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US 2021/0010073 A1Published application
Preparation of nucleic acid libraries from rna and dna
OwnerHongxia Xu, Dan Cao +1inventors
InventorsHongxia Xu, Dan Cao, Alex Aravanis
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Abstract
Some embodiments of the methods and compositions provided herein relate to the preparation and use of nucleic acid libraries derived from RNA and DNA. In some embodiments, a nucleic acid library can be prepared by tagging polynucleotides derived from RNA. Some embodiments include the analysis of sequence data from such libraries.
Claims21
-43. (canceled)
A method for preparing a library of nucleic acids comprising:
- (a) hybridizing a plurality of polynucleotides with a plurality of primers, wherein the plurality of polynucleotides comprises RNA and DNA;
- (b) extending the hybridized primers with a reverse transcriptase;
- and
- (c) generating a library of nucleic acids from the extended primers and the DNA.
1 more element
A kit for preparing a library of nucleic acids comprising:
- a reverse transcriptase lacking a DNA-dependent polymerase activity;
- a plurality of primers comprising tags, wherein each primer comprises the same tag, and each primer is different;
- and
- a component selected from the group consisting of a kinase, an RNase, a ligase, a transposon, a polymerase, and a sequencing adaptor.
1 more element
Dependencies are inferred from the claim text.