Lipid Screening Platform Allowing a Complete Solution for Lipidomics Research

A method for quantitating lipids of a matrix sample using a differential mobility spectrometry (DMS) device during a targeted multiple reaction monitoring (MRM) acquisition experiment, comprising:

• sequentially receiving a first injection and a second injection of a matrix sample and separating known lipid molecules of the matrix from the first injection and the second injection over time using a separation device, wherein before the experiment the known lipid molecules of the matrix are grouped into lipid classes and the lipid classes are further grouped into a pass-through group that includes lipid classes without lipid molecules known to produce isobaric interferences with other lipid molecules and a mobility separation group that includes lipid classes with lipid molecules known to produce isobaric interferences;
• receiving separated lipid molecules from the separation device for the first injection and the second injection and ionizing the separated lipid molecules for the first injection and the second injection using an ion source;
• receiving a first beam of ions of the separated lipid molecules for the first injection and passing the first beam through without ion mobility separation using a DMS device;
• receiving a second beam of ions of the separated lipid molecules for the second injection and sequentially mobility separating the second beam according to compensation voltage (CoV) values experimentally predetermined for each lipid class of the mobility separation group using the DMS device;
• receiving the first ion beam from the DMS device, for a first plurality of cycles, performing an MRM scan for at least one MRM transition for each lipid molecule of each lipid class of the pass-through group, and storing a first set of intensity values of each MRM scan for the first plurality of cycles using a tandem mass spectrometer;
• receiving the second mobility separated ion beam from the DMS device, for a second plurality of cycles, performing an MRM scan for at least one MRM transition for each lipid molecule of each lipid class of the mobility separation group, and storing a second set of intensity values of each MRM scan for the second plurality of cycles using the tandem mass spectrometer, wherein each MRM transition of each lipid molecules is experimentally predetermined;
• and
• receiving the first set of intensity values and the second set of intensity values, receiving MRM intensity and concentration values for known standards of the known lipid molecules of the matrix, and quantitating each lipid molecule in the matrix sample by comparing the first set of intensity values and the second set of intensity values to the MRM intensity and concentration values for the known standards of the known lipid molecules of the matrix using a processor.