Computer implemented discovery of antibody signatures
AssigneeCeredx Inc.
InventorsTaura L. Barr, Grant O'Connell
Life of this patent
Estimating the term…
Abstract
Disclosed herein are computer implemented methods of distinguishing ischemic stroke, hemorrhagic stroke, and stroke mimic by detecting circulating biomarkers in a subject. Also provided herein are systems, kits, and methods for the detecting.
Claims64
A method comprising performing, using a computer processor, a random forest analysis on a first sample and a second sample, wherein the first sample and the second sample are associated with an array, wherein the first sample comprises a stroke patient biological sample and the second sample comprises a stroke mimic patient biological sample, wherein the array comprises at least one protein probe;
- wherein the random forest analysis comprises:
- (a) comparing a binding intensity level of antibodies in the first sample with the at least one protein probe to a binding intensity level of antibodies in the second sample with the at least one protein probe;
- and
- (b) generating a gini impurity score between the first sample and the second sample for the at least one protein probe.
A method comprising:
- (a) contacting a sample with a synthetic protein;
- (b) detecting a binding intensity level of antibodies in the sample with the synthetic protein;
- and
- (c) comparing the binding intensity level to a reference, wherein the reference comprises a reference binding intensity level or a derivative thereof of antibodies in a stroke mimic sample with the synthetic protein.
A method comprising:
- (a) contacting a sample with one or more synthetic proteins, wherein the one or more synthetic proteins comprise an amino acid sequence at least 80% identical with any one of SEQ ID NO:1 to SEQ ID NO:50;
- and
- (b) detecting a binding intensity level of antibodies in the sample with the one or more synthetic proteins.
A kit comprising:
- (a) a synthetic protein comprising an amino acid sequence at least 80% identical to any one of SEQ ID NO:1 to SEQ ID NO:
- 50;
- and
- (b) a detecting reagent for detecting binding of an antibody with the synthetic protein.
A synthetic protein comprising an amino acid sequence at least 80% identical to any one of SEQ ID NO:
- 1 to SEQ ID NO:
- 50.
A method comprising:
- (a) contacting a sample with a synthetic protein, wherein the synthetic protein comprise an amino acid sequence at least 80% identical to any one of SEQ ID NO:1 to SEQ ID NO:
- 50;
- (b) detecting a binding intensity level of antibodies in the sample with the synthetic protein;
- and
- (c) comparing the binding intensity level to a reference.
The method of any one of claims 58-61, wherein the reference is a control.
The method of any of preceding claim, wherein the sample comprises a cell-free sample.
Dependencies are inferred from the claim text.