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AssigneeUniversity of Iceland, Center for Systems Biology
InventorsGiuseppe PAGLIA, Sirus PALSSON, Aarash BORDBAR
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Disclosed herein are methods, biomarkers, systems, compositions and kits for determining the phase or metabolic state (e.g. First Phase, Second Phase, or Third Phase) of a red blood cell (RBC) sample or for determining the phase or metabolic state (e.g., First Phase or Second Phase) of a platelet (PLT) cell sample. The methods disclosed herein are related to the use of isolated RBC sample or isolated PLT sample and analytical tools for providing information that is relevant to the phase or metabolic state of the RBC sample or the PLT sample. The system disclosed herein utilizes isolated RBC sample or isolated PLT sample and at least one analytical tool or an output from the at least one analytical tool. The compositions and kits described herein utilize RBC samples or PLT samples, including compositions in a form that allows for analysis of the RBC sample or the PLT sample.
1-73. (canceled)
A method of determining whether a blood sample is safe for transfusion into a subject, the method comprising:
The method of claim 1, wherein the testing module is operatively connected to an inner wall of a storage container configured to store the blood sample.
The method of claim 1, wherein the detecting the concentration of hypoxanthine is performed by the testing module using a colorimetric assay or a luminescent assay.
The method of claim 1, further comprising separating the blood sample into an extracellular portion and an intracellular portion with the reagents of the testing module, wherein the detecting the concentration of hypoxanthine comprises analyzing the extracellular portion of the blood sample.
The method of claim 1, wherein the blood sample is a red blood sample.
The method of claim 1, wherein the blood sample comprises red blood cells and an additive solution.
The method of claim 1, wherein the blood sample is an extracellular blood sample.
The method of claim 1, further comprising detecting a concentration of inosine, adenine, pyruvate, glucose, lactate, Na+, K+, or any combination thereof in the blood sample.
The method of claim 1, further comprising administering the blood sample to a subject in need thereof.
A method of administering a blood sample to a subject in need thereof, the method comprising:
The method of claim 10, wherein a ratio of hypoxanthine:adenine in the range of 0 to about 1.0 is associated with the First Phase, a ratio in the range of about 1.0 to about 16 is associated with the Second Phase, and a ratio greater than about 16 is associated with the Third Phase.
The method of claim 10, wherein a concentration of hypoxanthine in the range 0 mM to about 0.1 mM is associated with both the First Phase and the Second Phase and a concentration of hypoxanthine greater than 0.1 mM is associated with the Third Phase.
The method of claim 10, wherein assigning the metabolic state in (b) is performed using a colorimetric assay or a luminescent assay.
The method of claim 10, wherein the control comprises a signature profile of the one or more biomarkers over time.
The method of claim 10, wherein the one or more biomarkers further comprises a concentration of inosine, a concentration of adenine, a ratio of glucose:lactate, a ratio of Na+:K+, a ratio of pCO2:pH, a ratio of inosine:adenine, a concentration of pyruvate, or a combination thereof.
A method comprising administering a blood sample to an individual that is assigned a metabolic state of a First Phase or a Second Phase based on a level of one or more biomarkers measured in the blood sample, wherein the one or more biomarkers comprises hypoxanthine, or a ratio of hypoxanthine and adenine, or a combination thereof.
The method of claim 16, wherein the blood sample is assigned a metabolic state of a First Phase, a Second Phase, or a combination thereof if the level of hypoxanthine measured is from 0 mM to about 0.1 mM.
The method of claim 16, wherein the blood sample is assigned a metabolic state of a First Phase if the ratio of hypoxanthine:adenine measured is from 0 to about 1.0.
The method of claim 16, wherein the blood sample is assigned a metabolic state of a Second Phase if the ratio of hypoxanthine:adenine measured is from about 1.0 to about 16.
The method of claim 16, wherein the one or more biomarkers further comprise a concentration of inosine, a concentration of adenine, a ratio of glucose:lactate, a ratio of Na+:K+, a ratio of pCO2:pH, a ratio of inosine:adenine, a concentration of pyruvate, or a combination thereof.
Dependencies are inferred from the claim text.